RCSB PDB Data Validation and Deposition Services


SF-Tool | pdb_extract | Validation Server | ADIT deposition tool | ADIT-NMR | NEW!Try new ADITBeta! | NEW!Ligand Expo

Welcome to the RCSB PDB Deposition Portal. For other deposition servers (PDBj, PDBe), please visit the wwPDB page.
Returning users can begin by using the quick links above.


The following data deposition tools and instructions can make your structure deposition easy, complete and accurate:

  1. Check the announcements to be familiar with the latest format and deposition policy changes.
  2. Gather information about the proteins/nucleic acids in your structure from sequence databases (e.g. using UniProtKB 1 or BLAST 2 ) or about your ligand (using Ligand Expo, formerly Ligand Depot 3 ). If you wish to deposit a new ligand, instructions will be provided during deposition.
  3. Convert and Check your crystallographic structure factors with our SF-Tool, including SFcheck and sf-convert among other tools, if appropriate.
  4. Prepare your structural data for deposition using pdb_extract 4 and/or the desktop version of ADIT.

    5. THEN

  5. Validate your structure using our Validation Server. Structures solved by NMR are currently limited to model coordinate validations.
  6. Deposit your structure using the structure deposition tool ADIT or ADIT-NMR.

    OR

  7. Validate and Deposit with one tool: ADITBeta!

The Validation Server can also be used to monitor improvements made to your structural model before you begin the deposition process.

Detailed step-by-step instructions for individual structural methods are available.
  • Instructions for X-ray crystallography structure depositions.
  • Instructions for NMR structure depositions.
  • Instructions for EM structure depositions.
  • More information on deposition of structures determined by other methods
    (including Electron diffraction, Fiber diffraction).

Tutorials for first-time and experienced depositors:
 


RCSB PDB Data Deposition Tools at a Glance

Frequently Asked Questions About Depositing, Updating and Releasing Files


Policies and Procedures

Announcements


05/05/08 - We are happy to announce the release of the new RCSB PDB Deposition Portal!

Improvements to the layout and instructions make the deposition process easier to understand and complete, with deposition tool interfaces (pdb_extract, ADIT, etc.) also being updated shortly. We have also improved our Crystallographic Data Validation tool and turned it into SF-Tool, which offers online versions of sfcheck and our structure factor conversion tool, sf-convert, along with other useful tools specifically for X-ray structures.


05/05/08 - We are also happy to announce the next version of Ligand Depot, Ligand EXPO!

It is not only faster for you to find your ligand of interest, but you can easily search for related chemical components and interactively view the ligand within their PDB entries (click here for an example of caffeine (CFF); you must have Java enabled). A sketching tool is provided where you can upload a variety of 2D structure files (including REFMAC monomer sketcher library files) for conversion to mmCIF (or other) formats, and a new Browse feature that lets you search for and interactively view today's most important molecules.


During the annotation of structures the numbering and chain IDs of chemical groups and waters may be changed in accordance with the wwPDB chain ID rules. Thus deposition and processing of structures prior to preparation of the manuscript will ensure consistent usage of chain IDs and residue numbers in the manuscript and the PDB file.


Effective February 1, 2008, structure factor amplitudes/intensities (for crystal structures) and restraints (for NMR structures) are a mandatory requirement for PDB deposition. This policy is published at http://www.wwpdb.org/news.html.


Polypeptide structures containing 24 or more residues and polynucleotide structures with 4 or more residues are accepted by the wwPDB.


The status of all entries is updated every Tuesday at 12 noon EST. All new entries deposited during the previous week (before Friday 8am EST) will be searchable following the update. The searchable status information includes title, author names and deposition date. The current status of experimental data, coordinates, and sequence is also available.


If modifications (REVDATs) need to be made to entries released as part of the Remediation Project after August 1, 2007, the re-released files will follow Contents Guide Version 3.1.


Questions, comments, and suggestions should be sent to deposit@deposit.rcsb.org.



1 The UniProt Consortium. (2008) "The Universal Protein Resource (UniProt)." Nucleic Acids Res. 36:D190-D195.
2 Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) "Basic local alignment search tool." J. Mol. Biol. 215:403-410.
3 Feng, Z., Chen, L., Maddula, H., Akcan, O., Oughtred, R., Berman, H.M., & Westbrook, J. (2004) " Ligand Depot: a data warehouse for ligands bound to macromolecules " Bioinformatics 20:2153-2155.
4 Yang, H., Guranovic, V., Dutta, S., Feng, Z., Berman, H.M., & Westbrook, J.D. (2004) " Automated and accurate deposition of structures solved by X-ray diffraction to the Protein Data Bank. " Acta Cryst. D60, 1833-1839.